PHASE CONTRAST MICROSCOPIC OBSERVATIONS ON THE THIN SECTIONED TISSUE CULTURE

Low Temperature Ultramicroincineration of Thin-sectioned Tissue

Low temperature ultramicroincineration was employed to determine the morphological localization of "structure-bound" mineral and/or metallic elements within biological cells at the electron microscope level. This technique chemically removes organic material from thin sections of tissues with reactive, excited oxygen instead of heat as used in a furnace. The remaining ash representing the miner...

Phase-contrast and electron-microscopic observations on a membranous complex in primary spermatocytes of the mouse.

A prominent cytoplasmic inclusion present in living mouse primary spermatocytes has been observed by both light and electron microscopy. It began to form at prometaphase and continued to increase in thickness and length as the cells developed. By metaphase it was a distinct sausage-shaped boundary that enclosed a portion of the cytoplasm between the spindle and the cell membrane. At the end of ...

Observations on the use of live-modified tissue culture vaccine against Sheep Pox

Electron Microscopic Observations of Rat and Mouse Cerebellum in Tissue Culture

Closely ordered stages of myelin formation in cultures of newborn rat and mouse cerebellum, selected by direct light microscopy, were studied with the electron microscope. Electron micrographs of these cultures reveal the presence of neurons, axons, neuroglia, microglia, and ependymal cells. The appearance of the neuron is identical to that previously described in vivo. The neuroglial cell has ...

observations on the use of live-modified tissue culture vaccine against sheep pox

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